Journal: Life Science Alliance

Article Title: The NSL complex is required for piRNA production from telomeric clusters

doi: 10.26508/lsa.202302194

Figure Lengend Snippet: (A) Volcano plots showing the log 2 fold-change and P -value of all genes and transposons from the RNA-seq of NSL2 RNAi compared with white RNAi. Data shown are obtained from the DEseq2 analysis of three biological replicates. Shown in green are all genes and transposons with an absolute log 2 FC > 1 and a P -value < 0.05. Shown in red are all Group 1 transposons (from ) with the same requirements. (B) Barplot showing RT–qPCR fold changes for transposons HeT-A , TAHRE , blood, and burdock in the unfertilized eggs (pure germline RNAs) upon NSL2 RNAi (orange) compared with white RNAi (white). Values were normalized to rp49 . Values shown are an average of three biological replicates. Error bars represent SD. piwi P = 0.49; vasa P = 0.33 (paired t test). (C) Immuno-detection of the HeTA-Gag protein in the ovaries, a translation product of the HeT-A transposon, upon white RNAi and NSL2 RNAi. Scale bar, 10 μm. A representative image of n = 6 ovaries is shown. (D) Immuno-detection of γ-H2Av protein in white RNAi and NSL2 RNAi ovaries. Arrowheads indicate nurse cells showing accumulation of γ-H2Av. DAPI, 4′,6-diamidino-2-phenylindole. Scale bar, 10 μm. A representative image of n = 6 ovaries is shown.

Article Snippet: anti-γ-H2Av , mouse , 1:300 (IF) , UNC93-5.2.1 (DSHB).

Techniques: RNA Sequencing Assay, Quantitative RT-PCR

Journal: Life Science Alliance

Article Title: The NSL complex is required for piRNA production from telomeric clusters

doi: 10.26508/lsa.202302194

Figure Lengend Snippet: List of antibodies used for immunofluorescence staining (IF) and Western blotting (WB).

Article Snippet: anti-γ-H2Av , mouse , 1:300 (IF) , UNC93-5.2.1 (DSHB).

Techniques: Immunofluorescence, Staining, Western Blot